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KMID : 1094020120290050372
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Journal of Veterinary Clinics
2012 Volume.29 No. 5 p.372 ~ p.376
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Effective Biomarkers for Miniature Pig in Acute Kidney Injury Using Renal Ischemia-Reperfusion Model
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Kim Se-Eun
Shim Kyung-Mi
Kang Seong-Soo
Choi Seok-Hwa
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Abstract
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Acute kidney injury (AKI) is a serious problem associated with high morbidity and mortality. Ischemiareperfusion is an important cause of acute kidney injury. This study was performed to ascertain clinically useful biomarkers for the diagnosis of AKI. In three miniature pigs, AKI were induced by 60 minutes of bilateral renal ischemia by the clamping renal artery. Blood and urine samples were collected from the pigs prior to clamping (baseline) and 0, 1, 3 and 5 days post-clamping. Serum blood urea nitrogen (BUN), creatinine, sodium and uric acid were measured in serum and urine samples. Fractional excretion of sodium (FENa) and fractional excretion of uric acid (FEUA) were calculated. Also, interleukin (IL)-6, IL-18, liver type fatty acid binding protein (L-FABP) and glutathione-S-transferase (GST) were detected by Western immunoblotting. Serum BUN and creatinine levels were increased significantly at day 1 post-clamping in all three miniature pigs. However, FENa and FEUA showed marked individual differences. Western immunoblotting revealed significantly increased levels of IL-6, IL-18, L-FABP and GST in post-ischemic urine, compared to pre-clamping. While more research concerning the variance of FENa and FEUA is needed, serum BUN, creatinine, IL-6, IL-18, L-FABP and GST may be sensitive urine biomarkers for diagnosis of AKI together with other biomarkers in the porcine ischemia-reperfusion model.
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KEYWORD
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acute kidney injury, biomarkers, renal ischemia and reperfusion, miniature pig
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